Norovirus Extraction from Frozen Raspberries Using Magnetic Silica Beads

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Despite the warning labels that come on silica gel packets, these babies are actually pretty useful for keeping things dry!

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Conclusion

A method based on magnetic silica beads to extract HuNoV virus RNA from frozen raspberries was developed. The MSB method performance was similar to the reference method ISO 15216-1:2017. The influence of RT-qPCR inhibitors extracted using both methods was reduced using an alternative RT-qPCR detection kit (TaqMan Fast Virus 1-Step Master Mix) and condition. In future, the reduction of the RT-qPCR inhibitors which impact the HuNoV detection results should reduce the number of inconclusive assays and influence prevalence estimates.

Funding

This work was supported by the National Major Science and Technology Project for the Control and Prevention of Major Infectious Diseases of China (nos. 2018ZX10712001 and 2020ZX1001015), Beijing Municipal Science and Technology Project (no. Z181100001618015), the National Natural Science Foundation of China (nos. 81802049 and 81971979), and Beijing Key Clinical Specialty for Laboratory Medicine – Excellent Project (no. ZK201000).

Difference Between Silicon and Silica

Definition

Silicon: Silicon is an element found in the earth crust.

Silica: Silica is a compound made out of silicon and oxygen atoms.

Composition

Silicon: Silicon is a single element.

Silica: Silica is composed of silicon and oxygen atoms.

Melting Point

Silicon: Melting point of silicon is about 1414C.

Silica: Melting point of silica is about 1713C.

Electrical Conductance

Silicon: Silicon is a semiconductor which can conduct electricity.

Silica: Silica is not capable of conducting electricity.

Occurrence

Silicon: Silicon does not occur alone in nature.

Silica: Silica can occur as a pure compound.

Crystal Structure

Silicon: The crystal structure of silicon is brittle.

Silica: The crystal structure of silica is very hard.

Conclusion

Both silicon and silica contains silicon atoms. But silica is different from silicon due to their structures and properties. The main difference between silicon and silica is that silicon is an element whereas silica is a compound.

References:

1.”Chemistry of Silicon (Z=14).” Chemistry LibreTexts. Libretexts, 20 June 2017. Web. Available here. A30 June 2017. 2.”Silica.” Encyclopædia Britannica. Encyclopædia Britannica, inc., n.d. Web. Available here. 30 June 2017. 

Image Courtesy:

1. “SiliconCroda” By Enricoros at English Wikipedia – (Public Domain) via Commons Wikimedia2. “Zirconia-silica-beads-05mm-03” By Lilly_M – Own work (CC BY-SA 3.0) via Commons Wikimedia

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